In target gene-based inquiry mode, users select a species and input a gene using Gene Symbol, Entrez ID or Ensembl ID, then use FC≥2 & FC≤1/2 or FC≥3/2 & FC≤2/3 to filter T(co)F knockdown/knockout datasets in which the input gene is significantly expressed.

In target gene (BLAST)-based inquiry mode, users enter a gene sequence of interest, select the species and the degree of differential expression, KnockTF 2.0 can align the corresponding gene by running the BLAST program. Then, the gene-related T(co)F knockdown/knockout datasets can be displayed in the result page.