| Gene name: | ALPL |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Adipose |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Specifically, the number of SA-β-gal+ cells and the expression of p16 were obviously increased in the Alpl+/- BM, but TERT expression was decreased relative to that in the WT controls. |
| Target gene: | ATP |
| Official symbol(s): | ATP5MC1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | RadioActivate ATP assay |
| Target gene description: | After the induction, the Alpl+/- MSCs, but not the WT MSCs, released a large amount of ATP. More strikingly, the downregulation of Alpl led to increased extracellular ATP in the WT MSCs, whereas enforcing expression in the Alpl+/- MSCs significantly reduced the extracellular ATP level, suggesting that Alpl in MSCs probably regulates ATP release. |
| Regulatory pathway: | AMPKα |
| R-AG-Pathway: | -- |
| Official symbol(s): | PRKAA1 |
| Pathway experiment: | Western blot |
| Pathway description: | The supernatant of the Alpl+/- MSCs suppressed the expression levels of p-AMPKα and p-ACC in 293T cell lines, indicating that the higher extracellular ATP levels due to the Alpl deficiency could inactivate the AMPKα pathway. |
Annotation:
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