| Description: |
p38 MAPK is activated simultaneously with the HtrA1-enhanced cell senescence in MEFs.To confirm that HtrA1 activates cell senescence through the p38 MAPK cascade, we examined the effects of a p38 MAPK inhibitor, SB203580, on the induction of cell senescence by recombinant HtrA1,SB203580 completely abolished the HtrA1- stimulated expression of p21CIP1/WAF1 and p16INK4a. Similarly, induction of SA-β-galactosidase activity by recombinant HtrA1 was inhibited by SB203580,showing that HtrA1 enhances cell senescence through the p38 MAKP cascade. |