| Description: |
To answer whether blocking the PI3K-Akt pathway would aggravate cellular senescence of hSKPs, we performed SA-β-Gal staining on hSKPs cultured with or without LY294002 and Akt inhibitor VIII for 12 days. LY294002 (10 lM) and Akt inhibitor VIII (5 lM) decreased the portions of spheres with no staining and moderate staining (Type A and B) while increasing the portion of spheres with intense staining (Type C), which suggested they could accelerate hSKP senescence. MTS assay showed significant decrease in hSKP growth after LY294002 and Akt inhibitor VIII treatment for 48 h. |