| External factors: | CNF1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | 70% of HCT-116 cells showed increased SA-β-gal activity after exposure to CNF1 for 72 h, whereas less than 9% of untreated and polyploid cell-derived daughter cells were positive for SA-β-gal activity.To confirm CNF1-induced senescent arrest, we analysed the expression of cell-cycle checkpoints proteins and observed that the expression of p53, p21 and p16 in HCT-116 cells were significantly increased after 24 h exposure to CNF1, and further increased after prolonged exposure during 72 h as compared with untreated control cells. |
| Regulatory pathway: | p53//p21//p16 |
| R-EF-Pathway: | Upregulation//Upregulation//Upregulation |
| Official symbol(s): | TP53//CDKN1A// |
| Pathway experiment: | Western blot |
| Pathway description: | To confirm CNF1-induced senescent arrest, we analysed the expression of cell-cycle checkpoints proteins and observed that the expression of p53, p21 and p16 in HCT-116 cells were significantly increased after 24 h exposure to CNF1, and further increased after prolonged exposure during 72 h as compared with untreated control cells. |
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