| Description: |
Short-term exposure to low doseH2O2 elevated protein expression of three senescence biomarkers, p53, p21, and p16, whose levels were suppressed by SHQA in a dose-dependentmanner.Whereas,SHQA suppressed the SA-β-Gal positive cell abundance at all three tested concentrations. The SA-β-Gal positive cell percentages were around 24%, 18% and 12% when pretreated with 0.5, 1.0, or 2.0 μM SHQA, respectively. Culturing and passaging HUVECs in growth medium supplemented with 2.0 μM SHQA remarkably delayed the cellular growth arrest. When cultured in normal medium supplemented with DMSO, the growth of HUVECs was arrested at 23.9 PDL (population doubling), whereas the supplementation of SHQA extended the cell growth to 26.3 PDL.The SA-β-Gal positive cells were significantly lower in cells supplemented with SHQA at PDL24 (24%), compared to cells cultured with DMSO at PDL23 (51%). SHQA treatment also reduced the protein expression levels of p53, p21, and p16 in senescent cells. |