| External factors: | SRT1720 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot//Confocal microscopy |
| Description: | After treatment with SRT1720, cellular senescence was examined by SA-β-gal staining (senescent cells were stained blue) and examined by bright-field microscopy (original magnification ×200). Scale bar, 50 μM. Percentages of blue cells per 50 cells were calculated. HDFs were also exposed to SRT1720 (10 μM) for 1 h and then protein levels were analyzed by immunoblotting. |
| Target gene: | SIRT1 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | SIRT1 |
| Target gene experiment: | Western blot |
| Target gene description: | HDFs were exposed to SRT1720 (10 μM) for 1 h and then protein levels were analyzed by immunoblotting. |
| Regulatory pathway: | SIRT1-NF-κB |
| R-EF-Pathway: | Upregulation |
| Pathway experiment: | Western blot |
| Pathway description: | HDFs were subjected to immunoprecipitation analysis using SIRT1 antibody or control IgG, and then blotted with antibodies-recognizing ac-NF-κB, LC3, and Beclin-1. |
Annotation: