| External factors: | Synthetic glucocorticoids |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experimental category: | L |
| Tissue type: | -- |
| Cell name: | Osteoblasts,ostecytes,endothelial cells |
| PMID: | 33758201 |
| Experiment: | Flow cytometry//SA-β-gal activity assay//In situ assay//qRT-PCR |
| Description: | Flow cytometry indicates high-level activation of the p16INK4a promoter.Flow cytometry analysis showed that the total number of βGal+ cells and the percentage of SA-βGal-expressing endothelial cells increased.We found an increase in the number of SA-βGal +cells in primary and secondary spongiosa regions.Co-staining of bone tissue sections with SA-βGal and Emcn also showed markedly increased percentage of SA-βGal-expressing blood vessels in metaphysis in MPS-treated mice.The femoral bone tissue sections revealed a much greater number of tdTom+ cells in both primary and secondary spongiosa regions in MPS-treated mice.QRT-PCR analysis revealed that CD144+ cells from MPS-treated mice had reduced expression of cell proliferative marker Ki67. |
| Target gene: | ANG//KI67//P16//P53//HMGB1 |
| R-EF-Target gene: | Downregulation//Downregulation//Upregulation//Upregulation//Upregulation |
| Official symbol(s): | ANG//KI67//P16//P53//HMGB1 |
| Target gene experiment: | Flow cytometry//In situ assay//qRT-PCR |
| Target gene description: | QRT-PCR analysis revealed that CD144+ cells from MPS-treated mice had reduced expression of cell proliferative marker Ki67 and much higher expression of p16INK4a and p53.HMGB1 and Ki67 signal were reduced or completely lost in cells from MPS-treated mice.The absolute numbers of RANK+ and TRAP+ cells expressing ANG were significantly reduced in response to MPS treatment. |
| Regulatory pathway: | ANG-PLXNB2 |
| R-EF-Pathway: | Downregulation |
| Pathway experiment: | DNA FISH |
| Pathway description: | Intense positive signal of Rrna transcription was detected in Emcn+ blood vessel cells in metaphysis of vehicle-treated mice,whereas much less positive signal was detected in blood vessels of MPS-treated mice. |
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