| External factors: | Daturataturin A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Western blot//SA-β-gal activity assay |
| Description: | We found that senescence-related molecules p21, p53 (Ser392), and p53 (Ser15) were activated by DTA and inhibited in the presence of the autophagy inhibitor 3-MA, suggesting that autophagy induced by DTA was closely related to senescence activation. β-Galactosidase (SA-β-Gal) staining confirmed the relationship between HaCaT autophagy and senescence. DTA at 30 μM showed a significant role in inducing senescence (p < .01), with inhibition observed in the presence of 3-MA (p < .05) |
| Target gene: | NF-κB |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | NFKB1 |
| Target gene experiment: | Western blot//ELISA |
| Target gene description: | DTA 7.5, 15, and 30 μM inhibited the nuclear expression of NF-κB and its downstream PCNA in a dose-dependent manner. IL-6, IL-8, and TNF-α secretion were measured by ELISA, and 30 μM DTA displayed excellent anti-inflammatory effects. |
| Regulatory pathway: | PI3K-Akt-mTOR-IL17//p53-p21 |
| R-EF-Pathway: | Downregulation//Activation |
| Pathway experiment: | Cell scratch assay//Western blot |
| Pathway description: | In the cell migration experiment, DTA inhibited IL-17-induced accelerated migration in a dose- dependent manner. DTA inhibits the phosphorylation of downstream Akt and mTOR (mammalian target of rapamycin, mTOR).//We found that senescence-related molecules p21,p53 (Ser392) ,and p53 (Ser15) were activated by DTA. |
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