Aging Overview

External factors

External factors: Apremilast
Aging type: Prevent
Aging characteristic:
Category: Chemical compounds
Phenotype: Osteoarthritis
Experimental category: L
Tissue type: --
Cell name: ATDC5
PMID: 33448323
Experiment: SA-β-gal activity assay
Description: IL-17 stimulation induced a 3.7-fold increase of SA-β-gal activity, while 1 ?M apremilast reduced its activity to only 2.1-fold


Regulatory relationship

Target gene: SIRT1
R-EF-Target gene: Upregulation
Official symbol(s): SIRT1
Target gene experiment: RT-qPCR//Western blot
Target gene description: Compared to the non-treated cells, IL-17 reduced the mRNA level of SIRT1 to 53%, which was recovered to 92%, close to the baseline, in the presence of 1 ?M apremilast. It was also confirmed that apremilast rescued the protein level of SIRT1 which had been significantly reduced by IL-17. As revealed in Fig. 7A, in normal SIRT-expressing cells, apremilast significantly suppressed cellular senescence, however, its effect was almost abolished in SIRT1-silenced cells. The results from western blot analysis in Fig. 7B and C revealed that the inhibitory effect of apremilast on PAI-1 and p21 was abolished by the silencing of SIRT1.

Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.