| External factors: | SHEAD-CM,HGF,SCF |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//ROS Assay//Mitochondrial membrane potential assay//RT-qPCR |
| Description: | The passage 3 (P3) to passage 8 (P8) hBMSCs were cultured in the conditioned medium from SHED (SHED-CM). The percentage of senescent cells was evaluated by β-galactosidase staining. The effects of HGF and SCF on mitochondrial function were assessed by measuring the ROS and mitochondrial membrane potential levels. Relative mRNA expression levels of p16, p21, Nanog, and OCT4 in passage 3 (P3) and passage 8 (P8) hBMSCs cultured in SHED-CM (P8-SHED-CM) and treated with 100 ng/ml HGF (P8-HGF 100), 10 ng/ml SCF (P8-SCF 10), and the combination of 100 ng/ml HGF and 10 ng/ml SCF (P8-H+S). |
| Regulatory pathway: | PI3K-ERK-STAT3 |
| R-EF-Pathway: | Upregulation |
| Pathway experiment: | Western blot |
| Pathway description: | Western blot results of the expression of mitochondrial-relative proteins (Mfn1, Mfn2, SOD2, and Catalase) and the PI3K/AKT, Erk1/2, and STAT3 signaling pathway-related proteins in passage 3 (P3) and passage 8 (P8) hBMSCs cultured in SHED-CM (P8-SHED-CM) and treated with 100 ng/ml (P8-HGF 100), 10 ng/ml SCF (P8-SCF 10), and the combination of 100 ng/ml HGF and 10 ng/ml SCF (P8-H+S). |
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