| External factors: | ALA |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Breast cancer |
| Experiment: | Western blot//PCR |
| Description: | Cytosolic expression of HMGB1 was increased in the ALA and ALA+RT groups, whereas nuclear expression levels of HMGB1 were decreased in these groups compared to the control. Moreover, expression levels of p53 and activation of p38 mitogen-activated protein kinase (MAPK) and NF- κB were significantly increased in the ALA+RT group compared to the control. The expres- sion levels of IL-6, IL-8, and Cxcl1 were measured by qPCR to additionally examine the effect of ALA on cellular senes - cence in irradiated cells. Increased levels of the correspond- ing mRNAs were observed in the ALA+RT group compared to all groups. The expression of p21, a cyclin- dependent kinase (Cdk) inhibitor that induces senescence through cell cycle arrest, was significantly increased in the ALA+RT group compared to the other groups. |
| Target gene: | HMGB1 |
| R-EF-Target gene: | Downregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation |
| Official symbol(s): | HMGB1 |
| Target gene experiment: | Western blot |
| Target gene description: | Cytosolic expression of HMGB1 was increased in the ALA and ALA+RT groups, whereas nuclear expression levels of HMGB1 were decreased in these groups compared to the control. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: