| External factors: | Icariin (ICA) |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot//MTT assay//flow cytometry |
| Description: | Senescence of iMr-90 cells was assessed by senescenc e-associated-β-galactosidase (Sa-β-Gal) staining assay. cell viability, and the expression levels of p53/p21, sirtuin (SirT) 1/6 and p50/p65 were determined via the MTT assay and western blotting respectively. The changes in cell cycle arrest of IMR?90 cells were assessed via flow cytometric analysis. |
| Regulatory pathway: | SIRT1-NF-κB |
| R-EF-Pathway: | Upregulation |
| Pathway experiment: | Western blot |
| Pathway description: | The ratio of p-p53/p53 and p-p21/p21, and protein levels of cav-1, SirT1 and SirT6 were determined via western blotting. The effects of D?gal and ICA on the ratio of of p?p53/p53 and p?p21/p21, and protein levels of cav?1 determined via western blotting were semi?quantified. The effects of D?gal and ICA on the protein levels of SIRT1 and SIRT6 determined via western blotting were semi?quantified. |
Annotation: