| External factors: | JQ1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Oesophageal cancer |
| Experiment: | SA-β-gal activity assay//qPCR//Western blot//CCK-8 assay |
| Description: | KYSE450 cells were treated with JQ1 for 144 hours, and cellular senescence was monitored by SA-β-gal staining. mRNA levels of p21, p27 in KYSE450 cells treated with JQ1 for 72 hours using qPCR. Immunoblotting for BRD4 and p21 in KYSE450 after treatment with vehicle or JQ1 for 48, 72, 96 and 144 hours. GAPDH served as a loading control. Four oesophageal cancer cell lines were treated with indicated JQ1 for 72 hours. The cell viability was assayed by CCK-8. |
| Target gene: | BRD4 |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | BRD4 |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | Expression levels of BRD2, BRD3 and BRD4 in oesophageal cancer cell lines using real-time PCR. Protein levels of BRD4 in various oesophageal cancer cell lines using immunoblotting. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: