| External factors: | AgNP |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//SAHF//SASP//Western blot//Confocal microscopy//flow cytometry |
| Description: | SA-β-gal staining was performed and senescent cells were stained with blue color, the ratio of SA-β-gal positive cells was calculated per group. Fluorescence microscopy images of SAHF formation. Heatmap depiction of SASP factor. Immunoblotting of γ-H2AX, p21, p-H3S10, H3K4-m3, and total of H3.β-Actin was used as loading control. Alexa Fluor 488-labeled antibodies (green) and Cy3-labeled Tel C telomere probe were used to identifyγ-H2AX and telomere, respectively. Cell cycle analysis using flow cytometry. |
| Target gene: | COX2 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | COX2 |
| Target gene experiment: | Western blot//Immunofluorescence |
| Target gene description: | NULL |
| Regulatory pathway: | COX2-PGE2 |
| R-EF-Pathway: | Upregulation |
| Pathway experiment: | ELISA |
| Pathway description: | The PGE2 levels in the culture medium was detected after treatment with AgNPs at 3rd, 7th and 10th day. The addition of COX2 inhibitor led to the decrease of PGE2 level in the culture medium. |
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