Aging Overview

External factors

External factors: AgNP
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: HL
Tissue type: Lung
Cell name: MRC5
PMID: 32763567
Experiment: SA-β-gal activity assay//SAHF//SASP//Western blot//Confocal microscopy//flow cytometry
Description: SA-β-gal staining was performed and senescent cells were stained with blue color, the ratio of SA-β-gal positive cells was calculated per group. Fluorescence microscopy images of SAHF formation. Heatmap depiction of SASP factor. Immunoblotting of γ-H2AX, p21, p-H3S10, H3K4-m3, and total of H3.β-Actin was used as loading control. Alexa Fluor 488-labeled antibodies (green) and Cy3-labeled Tel C telomere probe were used to identifyγ-H2AX and telomere, respectively. Cell cycle analysis using flow cytometry.


Regulatory relationship

Target gene: COX2
R-EF-Target gene: Upregulation
Official symbol(s): COX2
Target gene experiment: Western blot//Immunofluorescence
Target gene description: NULL

Regulatory pathway: COX2-PGE2
R-EF-Pathway: Upregulation
Pathway experiment: ELISA
Pathway description: The PGE2 levels in the culture medium was detected after treatment with AgNPs at 3rd, 7th and 10th day. The addition of COX2 inhibitor led to the decrease of PGE2 level in the culture medium.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view