| External factors: | TBHP |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//RT-qPCR//Western blot |
| Description: | tBHP treated and control cells were stained for SA-β-galactosidase activity on D9 of the experiment. RT-qPCR was performed to check for p21 mRNA levels. Protein expression of phosphorylated pRB, p53, p-p53 (Serin15) and p21 was measured by Westernblot on D4 and D9 of the experiment. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Protein expression of phosphorylated pRB, p53, p-p53 (Serin15) and p21 was measured by Westernblot on D4 and D9 of the experiment. |
Annotation: