| External factors: | PTE |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//ELISA//Fluorescence//Flow cytometry |
| Description: | Taking advantage of cellular senescence models, we tested the possible effect of PTE and its main components on SA-β-gal staining. SA-β-gal-positive cells significantly increased in AAPH and H2O2 groups, while control, PTE, succinic acid, and adenosine groups were relatively low. |
| Target gene: | SIRT1//FOXO3A//BCL-2//AROS//HUR//BAX//P53//DBC1 |
| R-EF-Target gene: | Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Downregulation//Downregulation//Downregulation |
| Official symbol(s): | SIRT1//FOXO3A//BCL-2//AROS//HUR//BAX//P53//DBC1 |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | Compared with the AAPH group, the levels of Bcl-2 and Foxo3a mRNA expression significantly increased in control and PTE groups (10, 20, 40 μg/mL) (P<0.01), and the Bax level significantly decreased in those groups (P<0.01) (Fig. 6). Furthermore, compared with the H2O2 group, the levels of SIRT1, Bcl-2, and Foxo3a mRNA expression significantly increased in control, four batches of PTE, succinic acid, and adenosine groups, and the Bax level significantly decreased in those groups (P<0.01). We performed western blot assays to find out the effect of PTE on the protein levels of p53, HuR, AROS, and DBC1 in AAPH-induced cells. SIRT1 can regulate the expressionof FOXO3a and p53, and be regulated by DBC1 (known as KIAA1967), AROS (known as RPS19BP1) and the tumor suppressor HuR (known as ELA VL1). |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: