| External factors: | Exosome |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | TEM//SA-β-gal activity assay//Western blot//Flow cytometry |
| Description: | Western blot analysis confirmed that the isolated particles expressed exosome-specific markers: CD63, CD9, and Alixs. The particle size of ExoUMSCs was around 60–150nm as shown by dynamic light scattering (DLS). After OMSCs were incubated with Dil-labeled ExoUMSCs for 48h, fluorescence was detected inside the cells, indicating that ExoUMSCs?were efficiently internalized into the target cells. A number of β-gal-positive cells were significantly reduced in OMSCs after treated with ExoUMSCs. Expression of aging-related factors p53, p21, and p16 was also markedly reduced, and the level of Sirt1 was increased in the ExoUMSCs-treated OMSCs. The growth rate of OMSCs was significantly increased, and more OMSCs entered into S phase of cell cycle after treatment with ExoUMSCs. The numbers of EdU-positive OMSCs were significantly higher in the ExoUMSCs-treated OMSCs than the untreated cells. |
| Target gene: | MIRNA-136 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | MIRNA-136 |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | Among these miRNAs, miR-136 was the most abundant in UMSCs, as compared with OMSCs, and was increased more than 20-fold in OMSCs after cultured with ExoUMSCs, whereas no significant difference was observed for other miRNAs such as miR-106a, miR-155, and miR-29C among these groups. Moreover, the level of miRNA-136 in ExoUMSCs was also higher than that in ExoOMSCs. Next, we examined the role of miR-136 in regulating OMSC senescence and biological function. The mRNA and protein levels of senescent-associated markers p53, p21 and p16, as well as the β-gal+?cells, were markedly reduced in OMSCs after transfected with miR-136 mimics. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: