| External factors: | NPs |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//ELISA |
| Description: | The chiral effect of CuxCoyS NPs on the viability of senescent IMR-90 cells (induced by 0.1 μM doxorubicin (Dox) for 7 day) was studied. The successful construction of senescent cells was confirmed by the high expression of senescence-associated β-galactosidase and up-regulated levels of P16ink4a. Moreover, the high secretion levels of SASP factors such as IL 6 and IL 1, quantified by enzyme-linked immunosorbent assay (ELISA), further proved the presence of senescent cells The chiral effect of CuxCoyS NPs on the activities of senescent cells was investigated by incubating the NPs (L-, D-, and DL-CuxCoyS NPs) with senescent cells at different concentrations. The viability of senescent cells displayed an obvious decrease when incubated at the concentration greater than 60 nM, and the cells incubated with D-NPs showed poorest viability compared with the cells incubated with DL-NPs or L-NPs. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: