| External factors: | PTX |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Prostate cancer |
| Experiment: | SA-β-gal activity assay//WST-1 assay//Flow cytometry//ELISA |
| Description: | The levels of the β-galactosidase enzyme were deter- mined to measure senescence. Built on the premise that chemotherapy induces senescence, there is major logi- cal senescence in the DTX group, as reported, which shows a significant increase compared with the control group. For its part, the senescence values of the PTX group demonstrated lower values than UCG (Δ% = 35%), but are statistically and significantly lower compared with the DTX group, since it represents a Δ% = 77% (p < 0.05). The PTX + DTX group exhibited a smaller percentage of senescence than DTX, but slightly higher than the PTX-treated group.It presents an evident decrease in viability by combining DTX and PTX.It can be observed that PTX, mainly at 4 and 8 mM, by itself reaches levels of cytotoxicity comparable to those obtained with the combination of both drugs in particular to 48 and 72 h.The percentage of apoptosis was considerably higher in the groups treated with PTX?+?DTX or with PTX even in comparison with the DTX group.The DTX (Δ%?=?115%) and PTX (Δ%?=?225%) groups also demonstrated increased apoptotic activity. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: