| External factors: | High glucose |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Osteoarthritis |
| Experiment: | Western blot//SA-β-gal activity assay |
| Description: | The expression of senescence-related markers (p53, p21, and p16) increased markedly with increase in glucose concentration. SA-β-gal staining results revealed that HG significantly increased the number of SA-β-gal- positive cells in a concentration-dependent manner. |
| Regulatory pathway: | AMPK |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | AMPK |
| Pathway experiment: | Western blot |
| Pathway description: | Western blot results revealed that the ratio of p-AMPK/AMPK decreased in a concentration-dependent manner. Western blot analyses results revealed that HG decreased the phosphorylation level of AMPK, whereas AICAR increased the phosphor- ylation level of AMPK, suggesting that AICAR has the capacity to activate AMPK in HG induced chondrocytes. |
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