| External factors: | MATN3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Intervertebral disc degeneration |
| Experiment: | SA-β-gal activity assay//CCK-8 assay |
| Description: | SA-β-Gal staining assay showed that the antisenescence effect of USC-exos was mitigated, and it showed that SA-β-Gal staining-positive NPCs in USC shMATN3 -exos group increased compared to those in the USC conMATN3 -exos group but was still lower than the control group.The results revealed that the proliferation of NPCs was markedly promoted in response to USC-exos stimulation. |
| Target gene: | TGF-β |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | TGFB1 |
| Target gene experiment: | Western blot |
| Target gene description: | WB analysis of TGF-β canonical SMAD pathway and noncanonical pathway (AKT) activation. In the USCconMATN3-exos group, the level of TGF-β and the extent of p-SMAD, COL2, and ACAN expression were significantly increased. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: