| External factors: | Aesculus |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | The Cells that were only given 10 μM MIA stress showed considerable amount of aged cells as compared to control and A. indica preconditioned (10μg and 20μg) groups.A. indica preconditioning with (10μg and 20μg) resulted in increased viability and proliferation of cells as compared to stress group. |
| Regulatory pathway: | NF-κB |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | qPCR//ELISA |
| Pathway description: | The levels of TNF-α, IL-6, IL-1β, and MMP-13 mRNA expression increased 1.88, 2.89, 1.55, 1.21 and 8.30 folds in the stress group respectively as compared to the control group and A. indica treated groups. Similarly, the quantitative expression of Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) was measured by the mRNA level of NF-κB p65 and p50 (NF-κB 1) subunits in all the groups. The mRNA level of p65 and p50 markers increased 1.61 and 6.86 folds in the MIA induced stress group as compared to the control and A. indica treated groups.HADMSCs in control group and A. indica preconditioned groups (10μg and 20μg) showed substantially less IL-1β and TNF-α protein expression. |
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