| External factors: | 17β-E2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Western blot/SA-β-gal activity assay |
| Description: | In control siRNA-transfected HUVEC cells, H2O2 induces a remarkable increase in the phospho- ryaltion of Rb and 17b-E 2 significantly inhibits H2O2 s effect.17bE2 significantly inhibits H2O2-induced increase in the number of SA-b-gal-positive cells.(孟丽加的)17b-E 2 loses the capacity to thwart H2O2 -induced increase in the number of SA-b-gal- positive cells. |
| Target gene: | SIRT3 |
| R-EF-Target gene: | Upregulation |
| Official symbol(s): | SIRT3 |
| Target gene experiment: | RT-PCR//Western blot//Luciferase reporter assay |
| Target gene description: | As shown in the results from the real-time PCR, 17b-E 2 increases the expres- sion levels of SIRT3 mRNA in a dose-dependent manner in HUVEC cells.Immunot blot analysis shows that the protein expression levels of SIRT3 are elevated, when HUVEC cells are exposed to different concentrations of 17b-E 2. We con- structed a Luciferase-based reporter system to mea- sure the transcriptional activity of SIRT3. As expected, 17b-E 2 increases SIRT3 promoter activity and exhibits the maximum effect at the concentration of 0.1 μM. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: