| External factors: | CO |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot//RT-PCR |
| Description: | Exposure of WI-38 cells to H2O2 for 4 days significantly increased the number of SA-b-Gal-positive cells compared to the control group. However, treatment with CORM-A1 markedly reversed the number of H2O2-induced SA-b-Gal-positive cells. CORM-A1 significantly reduced the expression of TNF-a IL-6, p21, and PAI-1 in H2O2- induced senescent cells. |
| Target gene: | MIR-34A |
| R-EF-Target gene: | Downregulation |
| Official symbol(s): | MIR-34A |
| Target gene experiment: | RT-qPCR |
| Target gene description: | CORM-A1 strongly decreased the expression of miR-34a. |
| Regulatory pathway: | miR-34a-Sirt1 |
| R-EF-Pathway: | Downregulation |
| Pathway experiment: | SA-β-gal activity assay//RT-PCR |
| Pathway description: | CORM-A1 treatment significantly increased both mRNA and protein of Sirt1 levels.CORM-A1 strongly decreased the expression of miR-34a. CORM-A1 significantly decreased the number of miR-34a-overexpressing SA-b-Gal-positive cells. Notably, p21 and PAI-1 were upregulated by miR-34a overexpression, whereas CORM-A1 significantly reduced miR-34a-induced p21 and PAI-1 levels. Furthermore, the miR-34a-induced Sirt1 downregulation was reversed by CORM-A1. |
Annotation: