| External factors: | SP600125+IR |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Anaplastic thyroid cancer |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | We also observed that most of SP600125 plus IR-treated cells were still attached to dishes and had enlarged granular phenotype resembling senescent state.The number of SA-β-gal–positive cells was markedly increased when SP600125 was combined with irradiation, whereas singular treatment showed weak or moderate induction of SA-β-gal activity. Our previous study has demonstrated that the fraction of cell with high PKH-2 fluorescence and increased SSC represents senescent state (10).The combination treatment with both SP600125 and IR significantly increased the subpopulation of PKH-2hi/SSChi compared to singular treatment. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: