| External factors: | Normobaric mild hyperoxia |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Both HA- and NMH-treated cells exhibited the typical senescent cell morphology, such as cell enlargement, a reduced cytoplasm-to-nuclear ratio, saturation density, and increased sa-β-gal activity, after 7 days of culture. After this time point, the cells gradually displayed the characteristics of replicatively senescent cells in a time-dependent manner. About 50% blue-stained cells have been shown after 14 days of culture under both conditions. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: