| External factors: | High glucose |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | BrdU assay//Western blot//SA-β-gal activity assay//Cell morphological analysis |
| Description: | BMSCs cultured in HG exhibited a time-dependent reduction in cumulative PD and BrdU incorporation.Cells cultured in HG for 2 weeks already show increased p16 and p21. HG also induced a time-dependent increase in SA-βgal positive cells .By contrast, glucose at 25 mM induced morphological changes consistent with senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: