| External factors: | CD9-Lac/CaCO3/Rapa |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | HDFs were treated with free Rapa or CD9-Lac/CaCO3/Rapa for 72 h, followed by 3 passages before analysis because the proliferative effect of Rapa was found to be high after 3 passages.The number of HDFs (P23) positive for β-galacto sidase staining clearly decreased following treatment with Rap-a or CD9-Lac/CaCO3/Rapa, which can be attributed to mTOR inhibitory activities of Rapa.The population doubling time was signficantly reduced in free Rapa- or CD9-Lac/CaCO3/Rapa-treated HDFs (P23) when compared to untreated old HDFs. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: