External factors: | Gamma-tocotrienol |
Aging type: | Prevent |
Aging characteristic: |
Category: | Other |
Phenotype: | Aging |
Experiment: | Flow cytometry//qRT-PCR//SA-β-gal activity assay |
Description: | γ-Tocotrienol c-Tocotrienol significantly decreased (p,0.05) the percentage of senescent HDFs in the G0/G1phase. In contrast,c-tocotrienol significantly increased (p,0.05) the percentage of senescent HDFs in the G2/M phase.However,c-tocotrienol produced significant downregulations of MMP1 mRNA expression in pre-senescent and senescent HDFs compared to untreated cells (p,0.05).c-Tocotrienol produced significant(p,0.05) upregulations of COL1A1 in young, pre-senescent,and senescent HDFs. c-Tocotrienol also significantly upregulated ELN mRNA in young and senescent HDFs. c-Tocotrienol significantly downregulated IL6, CCND1 and RB1 mRNA expression in senescent HDFs (p,0.05)..The incubation of senescent cells with 70 mM c-tocotrienol did not alter the percentage of SA-β-gal-positive cells. |
Regulatory pathway: | -- |
R-EF-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation: