| External factors: | Ionizing radiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Based on the phase contrast pictures, we observed that the irradiated cells alone become flattened and enlarged. Incidences of cells with senescent morphological features seem suppressed in cells irradiated in the presence of the studied inhibitors. Using microscopy and a chromogenic substrate, we determined cellular senescence through visualization of SA-β-galactosidase activity. Obviously, MCF-7 cells irradiated with a dose of 6 Gy do not die but enter premature senescence. SA-β-galactosidasepositive cells amounted to 79 % compared to mock-treated cells 3 days postirradiation. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: