| Description: |
To study the impact of HR suppression on the efficacy of telomerase inhibitor, HR in OE33 cells was first inhibited by lentivirus-based shRNAs targeting RAD51. RAD51-knockdown was confirmed by western blotting and HR suppression by plasmidbased assay. Cell growth: relative to control (CS) cells, the viability of RAD51-suppressed (R), GRN163L-treated CS and GRN163L-treated R cells was reduced by 34±5%, 24±6% and 65±5%, respectively. Thus,inhibition of HR or telomerase significantly reduced proliferation rate in OE33 cells, and combined treatment was associated with a significant 41% increase in cell death, relative to GRN163L used alone.Thus, simultaneous suppression of telomerase and HR led to a significant 36% (12-fold) increase in apoptosis, relative to telomerase inhibition alone.Evidence of senescence: cells were also evaluated for bgalactosidase staining, a marker for senescence. A subset of cells subjected to both telomerase and HR suppression stained positive for β-galactosidase, indicating senescence. |