| External factors: | X-ray |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Western blot |
| Description: | Our data indicated that HPAEC underwent cellular senescence upon exposure to 10 Gy X-rays as determined by increased SA-β-gal activity, detected cytochemically as blue perinuclear staining at 4 days post-irradiation.X-ray-exposed HPAEC also exhibited changes in cell morphology, displaying unusually large cells and flattened cytoplasmic appearance compared to sham-irradiated controls. We also observed the upregulation of p21/waf1 within 3h post-irradiation, consistent with inhibition of the cell cycle in advance of senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: