| External factors: | γ-irradiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//BrdU assay |
| Description: | Microscopic analysis revealed that after induction of DNA damage, all five cell lines adopted a senescent phenotype with a large, flattened shape and multiple or enlarged nuclei, and enhanced the expression of SA-β-galactosidase.Quantification of BrdU-positive cells showed that DNA damaged cells strongly reduced the incorporation of BrdU,confirming the absence of S-phase activity. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: