| External factors: | Gamma irradiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Then, the percentage of SA-β Gal stained cells was determined at different time points over 5 days after irradiation treatment. The highest percentage of SA-β Gal stained MRC-5 fibroblast cells (63 ± 4 %) was noted after the highest irradiation dose (20 Gy) and the longest time lapse (120 h) [72]. Therefore, HFF strains were only irradiated by 20 Gy. After 120 h the percentage of SA-β Gal stained HFF cells was similar to the corresponding value for the MRC-5 fibroblasts. protein expression levels of p21 and p16 were found to be significantly up-regulated in irradiation induced senescent fibroblasts compared to controls. Unexpectedly, a number of cytokines and cytokine receptors (IL11, EGFR, CXCL-1,2,3,5,6,14) were significantly down-regulated on irradiation induced senescence in MRC-5 and HFF strains, resulting in a significant down-regulation of the KEGG pathway “cytokine–cytokine receptor interaction” (hsa04060) representing SASP. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: