| External factors: | Chromosome 5 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Melanoma |
| Experiment: | Telomerase activity assay//SA-β-gal activity assay |
| Description: | Assay of telomerase activity:A2058#5 clone showed a 90% decrease in telomerase activity，this indicates that normal human chromosome 5, at its physiological level, suppressed hTERT expression and telomerase activity in A2058 cells. SA-β-gal activity assay:A2058#5S clones presented with significant morphological changes compared to parental A2058 cells, characterized by an elongated and somewhat enlarged and flat shape,s, introduction of chromosome 5 greatly reduced the growth rate of A2058 cells. Additionally, the majority of A2058#5S clones entered cellular senescence at around 20 PDs, as determined by SA-b-Gal staining. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: