| External factors: | Hyaluronan |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Flow cytometry//SA-β-gal activity assay |
| Description: | MSCs cultivated in L-HA either at 0.16% or 0.5% concentration showed a significant reduction in cycling cells compared with the controls, and this was accompanied by a concomitant increase in quiescent cells.Flow cytometry analysis of MSC cultures evidenced that all the experimental conditions, but 0.16% HCC, modified the pattern of cell cycle profiles with respect to control conditions.The apoptosis process was not affected by incubation with the different HA mixtures.while enescence was noticeably reduced , as detected by annexin V flow cytometry analysis and in situ β-galactosidase assay.The number of MSC clones was reduced after incubation with all the analyzed HA mixtures. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: