| External factors: | Trabectedin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Sarcoma |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | DNA content histograms of SW872 (left) and U2OS (right) 24 and 48 hrs after treatment (10 nmol/l trabectedin). In both cell lines, trabectedin caused a marked G2/M arrest after 24 hrs, which was strongly enhanced by additional hyperthermia (upper row).treatment with additional hyperthermia prolonged trabectedin‐induced cell cycle arrest in both investigated cell lines (lower row). Hyperthermia alone caused no significant alterations in cell cycle distributions (data not shown).Particularly U2OS—instead of undergoing apoptosis—showed a strong senescence response and a heat‐dependent continuously increasing number of senescent cells over 72 hrs (data not shown) to 144 hrs. In comparison, SW872, which preferentially underwent apoptosis, showed no detectable induction of senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: