| External factors: | Convallatoxin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Lung cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis//Western blot |
| Description: | Exposure of A549 cells to CON at 10 and 100 nM showed a significant increase in cells in the G2/M phase at all times tested, followed by an increase in cells in subG1 over the time.We also evaluated the expression of a key molecular driver of G2/M phase, cyclin B1, after 24 h of treatment by WB analysis.CON down-regulates cyclin-B1. CON induced at 50 nM an increase of ~80% of β-gal-positive cells. At 10 nM, an increase of larger regular nuclei was observed as well as the cells become flat, increase their volume and display a vacuole-rich cytoplasm, characteristic of senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: