| External factors: | Valproic acid |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Prostate cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | Contrary to DU-145 cells, in PC-3 cells, a 24 h-exposure to 2 mM VPA caused a G2 cell cycle arrest.The light scatter plot shows enlargement and flattening of cells with increased granularity in the VPA-treated group.Induction of senescence (blue β-gal staining) and inhibition of proliferation (brown Ki-67 staining)simultaneously is depicted .By examining SA-β-gal activity we confirmed that VPA did induce cellular senescence in PCa. The greatest induction of senescence was in DU-145 cells exposed to 0.4% w/v VPA. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: