| External factors: | MLN4924 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Glioblastoma |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Western blot//Cell morphological analysis |
| Description: | Fluorescence-activated cell sorting analysis revealed that MLN4924 notably triggered G2/M cell-cycle arrest in GBM cells.MLN4924 induced significant accumulation of WEE1, whereas it decreased the expression of p-H3 sharply, indicating that G2 cell-cycle arrest was induced by MLN4924.During the investigation of mechanistic basis for the inhibitory effect of MLN4924, we observed that MLN4924 induced an enlarged and flattened cellular shape in U251 cells , which suggested that the cells underwent senescence. senescence-associated β-galactosidase (SA-β-gal; a classical biochemical marker of senescence) was determined by SA-β-gal staining in these cells. As expected, a substantial fraction of MLN4924-treated U251 cells were positively stained, which demonstrated that MLN4924 triggered senescence in U251 cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: