External factors: | LY2801653 |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Cholangiocarcinoma |
Experiment: | SA-β-gal activity assay//Western blot |
Description: | We have analyzed senescence by measuring the senescence associated β-galactosidase (SA-β-Gal) activity assay in TFK-1 and SZ-1 cells after treatment with DMSO and LY2801653. In both cell lines we observed a significant increase in the percentage of SA-β-Gal activity after 48 and 96h compared to their controls.Moreover P21 is one of the major players linking cell cycle arrest and induction of senescence . We observed a strong expression of P21 compared to the untreated controls under 10mM LY2801653 at 96h. |
Regulatory pathway: | -- |
R-EF-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation: