| External factors: | Cisplatin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Lung cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | Visual examination revealed that the cells surrounding the recovering clones displayed morphologic changes that are associated with senescence, namely increased cell size and flattened shape.Detectable by flow cytometry, increased forward (cell size) and side (cellular granularity) scatter intensity is an additional characteristic associated with senescence . At day 17 of the recovery phase, quantification of senescence-associated β-galactosidase (SA-β-Gal) activity revealed that the fraction of SA-β-Gal-positive cells was 3.5-fold higher after treatment #3 compared to treatment #1 . recovery of F/S-low fraction featuring a normal cell cycle distribution was maximally delayed after treatment #3 and the remaining F/S-high cells (day14 rec) were primarily arrested in the G2/M-phase. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: