| External factors: | BET inhibitor JQ1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | T-cell lymphoma |
| Experiment: | Flow cytometry//Cell morphological analysis |
| Description: | JQ1 inhibited CTCL proliferation and induced cell cycle arrest.Following 24 h of treatment with JQ1, marked increase in G1 phase and reduced S phase were observed in all cell lines compared to untreated cells. Senescence induces morphological and flow cytometric changes like enlarged cells with a vacuole-rich cytoplasm resulting in increased forward/side scatter values [2]. R1 was defined as SSC (Side Scatter)low FSC (Forward Scatter)low region and R2 was defined as SSChigh FSChigh region in viable cells as previously described [10]. Treatment of MyLa cells with JQ1 increased the cell number in the R2 region and decreased that in the R1 region. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: