Aging Overview

External factors

External factors: 19i
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Urothelial carcinoma
Experimental category: L
Tissue type: --
Cell name: HEK293,UM-UC-3,VM-CUB1,HBLAK
PMID: 30064501
Experiment: Flow cytometry//MTT assay//SA-β-gal activity assay//Cell morphological analysis
Description: Based on the results from the MTT assays, in the following experiments, cells were usually treated with 2 μM 19i or with 2.5 μM SAHA. Treatment with 2 μM 19i impaired the clonogenic potential of UC cells comparably to treatment with the pan-HDACi SAHA. This was also the case for HEK-293 cells, but HBLAK again were less sensitive .Efficacious concentrations of 19i elicited an increased fraction of cells in the G2/M phase in UC cells and in the non-urothelial HEK-293 cells.Over time, cells became larger and flatter, indicative of cellular senescence .Indeed, some 19i-treated cells, especially from the VM-CUB1 cell line, stained positive for senescence-associated β-galactosidase.


Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.