| External factors: | 4SC-202 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Urothelial Carcinoma |
| Experiment: | Flow cytometry//SA-β-gal activity assay//Colony formation assay//Western blot |
| Description: | Compared to SAHA, the effect of 4SC-202 on clonogenic growth was more durable, resulting in persistent inhibition even 48 h after treatment. In HEK-293 cells, clonogenicity was also more strongly inhibited by 4SC-202 treatment.Neither 4SC-202 nor SAHA significantly increased the fraction of senescent cells in UM-UC-3 and VM-CUB1 cells. An increased fraction of SA-β-Gal positive cells was instead observed in non-urothelial HEK-293 cells and especially in the urothelial HBLAK controls.Notably, induction of p21CIP1, a classical marker of cell cycle arrest induction by HDAC inhibitors, was less prominent with 4SC-202 compared to SAHA in VM-CUB1 and UM-UC-3 cells after 24 h treatment. Significant p21CIP1 induction was only detected in both UCCs after 48 h of 4SC-202 treatment. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: