| External factors: | Rapamycin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//EdU Assay//Flow cytometry//Western blot//TUNEL assay |
| Description: | Cells treated with rapamycin demonstrated lower EdU incorporation, hence lower proliferation rate, compared to control cells.There were higher cells in G0 cell cycle in rapamycin treated cells compared to the control. Cells treated with rapamycin demonstrated significantly lower number of senescent cells compared to the control group at three weeks .Likewise, the expression of senescence marker p16 was lower in the rapamycin treated cells by Western blot.The results indicted a lower percentage of apoptotic cells in rapamycin treated HCEC compared to control. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: