| External factors: | 5-Fu |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Hepatocellular carcinoma |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | We acquired only SMMC?772 cells treated with rapamycin and 5?Fu in combination exhibited the distinctive morphology of senescent cells and were positive for senescence?associated β?galactosidase(SA?β?Gal), a hallmark of senescent cells24 .5?Fu treatment on SMMC?772 cells caused a significant loss of the cell cycle S/G2/M phase, addition of rapamycin induced a G arrest in SMMC?772 cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: