| External factors: | WS-CoQ10 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Alzheimer's disease |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | After culturing PSAF for over 27 passages, cells with no supplementation of PTS or WS-CoQ10 started to exhibit a wide cellular morphology, a sudden decrease in growth, and a high percentage of cells that were positive for blue SA-β-galactosidase (SA-β-gal) stain, a marker of cellular senescence. Slightly fewer SA-β-gal positive cells were found in the PTS supplemented PSAF and PSAF cultured with WS-CoQ10 had the lowest percentage of SA-β-gal stained cells.ad the lowest percentage of SA-β-gal stained cells.In conjunction to these findings, decreased levels of MnSOD, p21, Rb, and p16Ink4A were found in WS-CoQ10 cultured PSAF compared to cells without supplementation . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: