| External factors: | Resveratrol |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Squamous cell carcinoma |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//DAPI staining//Immunofluorescence//Western blot |
| Description: | We show that A431 cells treated with RES at concentrations between 10 and 50 μm for 48 h became enlarged and flattened and showed increased activity of SA-β-gal, a marker of senescence induction. On the other hand, most of the untreated control cells were SA-β-gal-negative. The SA-β-gal activity increased in a dose-dependent manner. The punctate nuclear accumulations of HP1γ and PML and the pancytoplasmic distribution of mtHsp70, which are known to be associated with senescence, were evident in RES-treated A431 cells . Senescence-associated features were also observed in other human SCC cells, such as SCC-13. However, confluence-induced quiescent cells were not SA-β-gal-positive (data not shown).The SA-β-gal activity also increased gradually over time in response to RES (30 μm) treatment.The dose- and time-dependent induction of p21WAF1, a cell cycle inhibitor associated with senescence, was detected in RES-treated A431 cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: